【文獻標(biāo)題】Novel insights into cytochrome P450 enzyme ａnd solute carrier families in cadmium-induced liver injury of pigs 【作者】Huan Wang, Qi Han,Yongjie Chen,et.al
【作者單位】東北農(nóng)業(yè)大學(xué)（Northeast Agricultural University）
【文獻中引用產(chǎn)品】
豬丙氨酸轉(zhuǎn)氨酶(ALT)ELISA試劑盒
豬天門冬氨酸氨基轉(zhuǎn)移酶（AST)ELISA試劑盒
【關(guān)鍵詞】Cadmium ,Cytochrome P450 enzyme family ,Solute carrier family ,Pig ,Transcriptomics
【DOI】https://doi.org/10.1016/j.ecoenv.2021.111910
【影響因子(IF)】6.2
【出版期刊】《Ecotoxicology ａnd Environmental Safety 》
【產(chǎn)品原文引用】
Serum isolated from anterior vena cava blood was used to determine alanine transaminase (ALT) ａnd aspartate transaminase (AST) activities by Microplate assay kits (Shanghai Hengyuan Bioengineering Co., Ltd., China) according to manufacturer’s protocol. Serum ALT ａnd AST levels were determined by double antibody sandwich method. First, the purified ALT or AST antibody of pigs was coated with the microporous plate, ａnd then serum was added to the micropore to form the antibodyantigen-elisa antibody complex. After thorough washing, substrate TMB was added for color development. The OD values were measured by an enzyme-labeled instrument at the wavelength of 450 nm. The concentration of ALT or AST in the sample was calculated by the standard curve.
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